Add time:08/14/2019         Source:sciencedirect.com

Saturated and mono- and di-unsaturated aldehydes (C5C10) were converted into their 2,4-dinitrophenylhydrazones. The separation of nanogram amounts of these hydrazones was performed by high-performance liquid chromatography with C18 reversed-phase columns in an isocratic elution. Separation of all aldehydes tested could noly be achieved at an elevated temperature (50°C). A linear relationshi between log k′ and the carbon number for a series of homologous compounds could be observed also at an elevated temperature. An optimal separation was achieved utilizing the methylene group selectivity which was found to be temperature dependent.Applicatin of this method shows the advantage of an optimized separation for the determination of carbonyl compounds from the oxidation of fatty acids in meat.

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