Add time:08/14/2019         Source:sciencedirect.com

A selective analytical method based on high-performance liquid chromatography, combined with atmospheric pressure ionisation mass spectrometry, was developed for the detection of atractyloside. The analysis was performed on an Xterra Phenyl column utilising a gradient elution profile and a mobile phase consisting of 10 mM aqueous ammonium acetate buffer–methanol–acetonitrile. The calibration curve of the method (1 ng/ml–160 μg/ml) was best described by a second order polynomial function (r2 = 0.998) but displayed good linearity in the range of 100 ng/ml–1 μg/ml (r2 = 0.999). The limit of detection for the atractyloside standard was determined and found to be 100 pg/ml and the limit of quantification of atractyloside in tuber matrix was found to be 250 pg/ml. The relative standard deviation of the method was on average below 5% (n = 8). The method was successfully applied to the analysis of Callilepis laureola tubers and unknown powdered samples for the presence of atractyloside.

We also recommend Trading Suppliers and Manufacturers of ATRACTYLOSIDE SODIUM SALT (cas 100938-11-2). Pls Click Website Link as below: cas 100938-11-2 suppliers