Add time:08/15/2019         Source:sciencedirect.com

A spermine-dependent, polyphenylalanine-synthesizing cell-free system having an optimum activity at 75–85°C, has been developed from the extremely thermoacidophilic archaebacterium Caldariella acidophila. The C. acidophila system is totally insensitive to the EF-Tu targeted antibiotics Pulvomycin (cas 11006-66-9) (at 40°C) and kirromycin (at 47–72°C) contrary to control systems derived from both mesophilic (Escherichia coli) and thermoacidophilic (Bacillus acidocaldarius) eubacteria. The archaebacterial EF-Tu-equivalent factor is also immunologically unrelated to eubacterial EF-Tu and does not cross react with antibodies against Escherichia coli EF-Tu. The pulvomycin and kirromycin reactions thus provide new phyletic markers for archaebacterial ancestry.

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