Add time:08/16/2019 Source:sciencedirect.com
Angiogenesis is a process of formation of new small blood vessels from existing vessels and is very critical for proper and rapid tissue healing. As reactive oxygen species (ROS) are known for their ability to promote angiogenesis, in this study zinc oxide (ZnO) and Zinc peroxide (cas 1314-22-3) (ZnO2) nano-particles (NP's) encapsulated chitosan (CS) and cellulose based hydrogels were synthesized. ZnO2 is comparatively stronger oxidizing agent as compared to ZnO, therefore, in this study it was hypothesized that ZnO2 would deliver better angiogenic potential than ZnO. Three types of hydrogels were prepared; control hydrogel (without nano-particles), hydrogel having ZnO NP's and hydrogel having ZnO2 NP's. The hydrogels were characterized by scanning electron microscopy (SEM) for structural morphology, Fourier transform infrared spectroscopy (FTIR) for chemical functional groups analyses and X-ray Diffraction (XRD) to investigate the crystalline or amorphous structure of NP's. The solution absorption capacity was tested in PBS and degradation was investigated in PBS, PBS/H2O2 and PBS/lysozyme solutions. The angiogenic behavior of these materials was studied in chorioallantoic membrane (CAM) assay and ZnO2 based material showed significant higher angiogenesis.
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