Add time:08/19/2019         Source:sciencedirect.com

Two symmetrical double-sided Schiff base ligands, H4L1 and H4L2, were synthesized by condensation of salicylaldehyde and 2-hydroxy-1-naphthaldehyde with oxalodihydrazine, respectively. The crystal structures of their vanadium complexes were analyzed by single crystal X-ray diffraction. The interaction between the synthesized complexes and BSA was studied by various spectral methods. The binding parameters including quenching constant KSV, binding constant K, and binding mode were obtained using fluorescence quenching method. The study shows that the quenching type was a static quenching process caused by formation of complex. The SOD activities of the complexes were determined by xanthine oxidase-NBT reduction method. The antioxidant capacity of the complexes was determined by the ABTS method. The results indicate that the activity of hybrid protein was more pronounced than that of the corresponding complex. At the same time, the hybrid protein can catalyze asymmetric oxidation of methyl phenyl sulfide.

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