Add time:08/17/2019         Source:sciencedirect.com

Seafood consumption is widely considered as the primary route for human exposure to the neurotoxin methylmercury (MeHg) that is produced by certain anaerobic microorganisms and can bioaccumulate to high concentration levels in natural aquatic food webs. In this study, a novel methylmercury-binding peptide with seven amino acids was displayed on the cell surfaces of Escherichia coli strain W-1, which was isolated from fish feces and fused with ice nucleation protein. These cells exhibited high affinity and selectivity toward methylmercury. They efficiently removed more than 96% of 12 μM methylmercury, and accumulation of methylmercury in the engineered strain was four times higher than that in the wild type. Transmission electron microscopy confirmed methylmercury accumulation on cell membranes. Carassius auratus was fed by engineered bacteria, which showed a decrease in methylmercury concentration in muscles of about 36.3 ± 0.7%; whereas an increase in methylmercury concentration was observed in the feces (36.7 ± 0.8%) in comparison to the control group. The engineered strain in the gut captured methylmercury and prevented it’s absorption by muscles, while some bacteria with methylmercury were excreted in the feces. The surface-engineered E. coli effectively protected fish from methylmercury contamination.

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