Add time:08/24/2019         Source:sciencedirect.com

This study describes the application of capillary electrophoresis (CE) to the analysis of Ebrotidine (cas 100981-43-9) and its metabolites as an alternative analytical technique to liquid chromatography. Comparison between UV–diode array spectroscopy and mass spectrometry (MS) using an ion-trap system with electrospray ionization as detection systems has been performed. The quality parameters of the UV detection method were established, obtaining linear calibration curves over the range studied (8–200 mg ml−1), limits of detection between 3.4 and 4.3 μg ml−1, and run-to-run and day-to-day precision lower than 14%. For these compounds the protonated species [M+H]+ and, in some cases, sodium adducts were observed in the MS spectra. Using MS coupled to CE, limits of detection were between 0.5 and 2.6 μg ml−1.

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