Add time:08/16/2019 Source:sciencedirect.com
Agnuside despite its good properties for the body, can be harmful to the kidneys in extra concentrations. So, its measurement is important. For the first time, we developed a simple, rapid and inexpensive method for the investigation of agnuside electrochemical behavior by designing a gold/reduced graphene oxide/carbon paste electrode (Au/RGO/CPE). The Au/RGO nanocomposite was synthesized through a one-pot in situ reduction of graphene oxide (GO) and HAuCl4 in the presence of sodium citrate and the obtained product was studied by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The anodic oxidation of agnuside has been carried out at a modified carbon paste electrode in 0.1 M phosphate buffer solution (pH 6.0) using sensitive differential pulse voltammetry (DPV). Several parameters such as the type and pH of supporting electrolyte and scan rates were optimized. This study reported a good linearity in the range of 0.050–250.0 nM and a low detection limit of 0.016 nM. The relative standard deviations (RSDs) for 10.0 nM and 100.0 nM of agnuside were obtained 3.3% and 1.2%, respectively. The sensor was successfully applied to determine agnuside in Vitex agnus castus fruit and urine sample.
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