Add time:08/24/2019         Source:sciencedirect.com

Bacosides, well-known brain tonic and restorative, are the active principles extracted from Bacopa monnieri. In the present investigation, bacoside A production by the hairy roots of B. monnieri was enhanced by optimizing the media components. Glucose as carbon source and potassium nitrate as nitrogen source yielded higher biomass and bacoside A production. Initially, medium components were selected using Plackett–Burman (PB) design and subsequently optimized using response surface methodology (RSM). Glucose, KNO3, KH2PO4 and MgSO4·7H2O at a concentration of 41.72 g l−1, 6.12 g l−1, 0.33 g l−1 and 0.52 g l−1, respectively, yielded maximum biomass and bacoside A production. Twofold increased in biomass (from 6.8 g l−1 FW to 12.99 g l−1 FW) and bacoside A production from 10.02 to 16.14 mg g−1 DW was achieved after optimization of conditions and medium components. Present study results suggested the success of RSM in enhancing the production of bacoside A and biomass accumulation of hairy root culture of B. monnieri.

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