Add time:08/20/2019         Source:sciencedirect.com

The pheromone trisporic acid induces zygophore development in zygomycetes. We study the interaction of the parasite Parasitella parasitica with one of its hosts, Absidia glauca. In analogy to the mating reaction, the (+) type of P. parasitica specifically infects the (-) type of A. glauca. Therefore, physiological relationships between the sexual pathway and the recognition between host and parasite may be postulated. In order to gain access to these phenomena at the molecular level, we have isolated cDNA clones, derived from mRNAs which are specifically regulated by trisporic acid. Mycelia from P. parasitica were sprayed with trisporic acid, and mRNAs were isolated 4.5 hours later. Following in vitro translation, we analyzed the corresponding protein patterns on two-dimensional gels. Under these conditions, only minor changes are caused by trisporic acid treatment. The most prominent ones are two additional spots close to pH 6 with a molecular mass of approximately 20 kDa. From a cDNA library derived from this mRNA, we isolated three cDNA clones, which show a regulated pattern in Northern-blots. Two clones hybridize with two size classes of mRNA, one of which is regulated, whereas the second one seems to be transcribed constitutively. In Southernblots with genomic DNA, two of the three cDNAs hybridize with repetitive DNA, the third one has at least two chromosomal copies.

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