Add time:08/16/2019         Source:sciencedirect.com

1.1. TRH and acid TRH were submitted to pyroglutamate amino peptidase digestion in vitro. The resulting cyclized compound corresponding to diketopiperazines of His-Pro were identified on paper electrophoregrams.2.2. The pseudo-hormones: Pyroglutamyl-Histidyl-Amphetamine, Pyroglutamyl-Histidyl-prolyl-Amphetamine, Pyroglutamyl-Amphetamine, Pyroglutamyl-Histidyl-O-Me were submitted to pyroglutamate amino peptidase digestion and the enzymic digests revealed the presence of free amphetamine. The result indicated that pyroglutamate aminopeptidase not only released N-terminal pyroglutamic acid but continuously hydrolyzed, step-wise the next amino acid probably as a result of amino peptidase contamination of animal liver origin.3.3. The prolinamides, Prolyl-Amphetamine and Pyroglutamyl-Histidyl-Prolyl-Amphetamine, were found independently to resist prolinase digestion.

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