Add time:08/20/2019         Source:sciencedirect.com

The specificity of frameshift mutatiosn induced by several classes of chemical mutagens was determined using a collection of mutant E. coli lacZ genes. This collection can detect each of five kinds of specific frameshift events by scoring Lac+ revertant colonies. In addition, the mutational spectra were characterized in backgrounds carrying plasmids that encode the umuDC, mucAB, or samAB operon. 4-Nitroquinoline 1-oxide (4-NQO) and furylfuramide (AF-2) induced efficientyly −1G, −2(C-G), and +1A frameshift mutations. 4-NQO and AF-2 differed in the ability for the induction of −1A and −1G frameshifts. +1A and −1A frameshift mutations induced by 4-NQO or AF-2 were enhanced by the introduction of the mucAB plasmid, and, to a lesser extent, the umuDC plasmid. The enhancing effect of the umuDC or mucAB plasmid on −1G and −2(C-G) frameshifts was weak or else not observed. 9-Aminoacridine was a potent inducer of +1G, −1G, and −1A frameshifts, whereas ICR-191 induced all types of frameshift mutations. A mutation enhancing effect was observed only on ICR-191-induced +1A frameshift mutations by the introduction of the mucAB plasmid. Mitomycin C caused no appreciable induction of frameshift mutations to the tester strains without plasmid. However, all types of frameshift, except −1G, were induced in the strains carrying the mucAB plasmid. N-methyl-N′-nitro-N-nitrosoguanidine induced all types of frameshift mutations. The mucAB plasmid enhanced mutagenesis in strains designed to detect the addition or loss of A · T base pair, indicating that the formation of +1A and −1A frameshifts was partly dependent on an error-prone SOS repair. Any frameshift mutagenesis was not affected by the samAB plasmid. In general, frameshifts in adenine runs were enhanced more preferentially by the mucAB and umuDC plasmids than frameshifts at runs of guanine were.

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