Add time:08/23/2019 Source:sciencedirect.com
A study was made of the TLC properties of the 3-beta-substituted esters, ethers, carbonic acid esters and halogen derivatives of cholesterol.As regards the TLC properties it can be stated as a general correlation that the same solvent systems are suitable for the separation of these groups of compounds (with the exception of the halogen derivatives). As a result of our experiment, four such systems could be found among many others tried.A regularity can be observed with regard to the polarity change of the esterifying component from C6 to C16 in the cyclohexane-chloroform (6:4) system.The cholesterol 3-beta-ethers are more polar than the esters, but with the lengthening of the etherifying side-chain the polarity decreases more rapidly than in the case of the esters; from the point of a TLC separation technique this is a very advantageous property.As regards their properties the cholesterol carbonic acid derivatives can be correlated with the cholesterol ethers.Compared with the above groups the 3-beta halogen cholesterol derivatives exhibit different properties. Their separation can be achieved with apolar solvents.
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