Add time:08/26/2019         Source:sciencedirect.com

A method was developed to increase the yield of MCL-PHA from Nonanoic acid (cas 112-05-0) in the PHA accumulation phase. Pseudomonas putida KT2440 was grown on glucose until ammonium-limitation was imposed. In the second (accumulation) stage, either glucose, nonanoic acid, or a mixture of these carbon and energy sources was supplied. Since the medium-chain-length poly-3-hydroxyalkanoate (MCL-PHA) subunits produced are unique for each carbon source, their relative contribution to PHA yield could be calculated. Y(C7+C9)/NA was 0.254 mol mol−1 during PHA synthesis from nonanoic acid. Y(C8+C10)/G was only 0.057 mol mol−1 during PHA synthesis from glucose. When nonanoic acid and glucose were fed together, Y(C7+C8)/NA almost doubled to 0.450 mol mol−1 while Y(C8+C10)/G decreased to 0.011 mol mol−1. These results demonstrate that substantial savings can be obtained by feeding glucose with substrates that are good for PHA production but much more expensive than glucose.

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