Add time:08/28/2019         Source:sciencedirect.com

The applicability of capillary electrophoresis for the separation of benzylpenicillin (penicillin G, PG) and its related substances was investigated. The CE method proposed uses sodium phosphate 40 mM–sodium dodecyl sulfate 150 mM, pH 6.5, as a buffer. This method is as selective as that of existing liquid chromatography (LC) methods. The limit of detection was 10 pg for PG and the limit of quantitation was 20 pg. PG samples were analysed by CE as well as LC. The analysis of variance showed no significant difference between the results obtained by the two techniques.

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