Add time:08/25/2019         Source:sciencedirect.com

An improved synthesis of the epinephrin metabolite, 4-hydroxy-3-methoxy-dl-madelic acid (HMMA), is described starting with 2-methoxyphenol and glyoxylic acid. The azo dyes derived from HMMA by condensation with the diazonium salts of p-nitroaniline and of sulphanilic acid have been prepared and characterized and are shown to be identical with the corresponding azo dyes derived from 2-methoxyphenol. The elimination of an oxyacetic acid group from HMMA occurs in the course of azo dye formation and may lead to ambiguities in the identification and quantitation of HMMA and other phenolic compounds in biological materials.

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