Add time:08/21/2019 Source:sciencedirect.com
A principal unidentified compound in the free ecdysteroid fraction and its conjugated form were purified from c.3 kg of ovaries of the silkworm, Bombyx mori, mainly by means of thin-layer chromatography and high-performance liquid chromatography. The purified compounds were identified as 22-DEOXY-20-HYDROXYECDYSONE (cas 19536-24-4) (22d20E) and 22-deoxy-20-hydroxyecdysone 3-phosphate (22d20E3P) by means of mass spectrometry and nuclear magnetic resonance spectroscopy. Although 22d20E had previously been isolated from the leaves and stems of the yew tree, Taxus cuspidata, and from the whole bodies of the sea spider, Pycnogonum litorale, it had not yet been obtained from insect ovaries. 22D20E3P was newly identified in the present study.
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