Add time:08/22/2019 Source:sciencedirect.com
Isolated hepatocytes from fed rats were exposed for 120 min to d-[1-13C]fructose, d-[2-13C]fructose, or d-[6-13C]fructose in the presence of H2O or D2O. The identification and quantification of 13C-enriched metabolites (d-glucose, l-lactate) in the incubation medium and the measurement of their deuterated isotopomers indicated that the ketohexose was phosphorylated predominantly at the intervention of fructokinase and that the majority of the d-glyceraldehyde molecules generated from d-fructose 1-phosphate were further metabolized, e.g., after phosphorylation to d-glyceraldehyde 3-phosphate. It is proposed that the present procedure may help to further characterize the regulation of d-fructose metabolism in both hepatocytes and other cell types.
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