Add time:07/18/2019         Source:sciencedirect.com

Azide- and amine-substituted sugars can be useful tools in the probing of biological systems as well as in the assembly of libraries of derivatives using click chemistry or simple amine coupling approaches. A collection of methylumbelliferyl glycosides of various azido- and amino-deoxy sugar derivatives of glucose, galactose and xylose was synthesised via azide displacement of the corresponding triflate derivatives and subsequent modification. These compounds will be used as substrates in a high-throughput screen to identify glycosidases that can process such modified sugars. The α-glycosyl fluoride derivatives of each modified sugar were also synthesised to serve as substrates for glycosynthases derived from the enzymes identified in the screen.

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