Add time:08/24/2019         Source:sciencedirect.com

We discovered the phenyllactate (PLA)-producing fungal strain Wickerhamia fluorescens TK1 and purified phenylpyruvate reductase (PPR) from fungal cell-free extracts. The PPR used both NADPH and NADH as cofactors with more preference for the former. The enzyme reaction as well as the fungal culture produced optically active d-PLA. The gene for the PPR (pprA) was cloned and expressed in Escherichia coli cells. Purified preparations of both native and recombinant PPR used hydroxyphenylpyruvate, glyoxylate and hydroxypyruvate as substrates but not pyruvate, oxaloacetate or benzoylformate. The predicted PPR protein had sequence similarity to proteins in the d-isomer-specific 2-hydroxyacid dehydrogenase family. Phylogenetic analyses indicated that the predicted PPR protein together with fungal predicted proteins constitutes a novel group of glyoxylate/hydroxypyruvate reductases. The fungus efficiently converted phenylalanine and phenylpyruvate to d-PLA. These compounds up-regulated the transcription of pprA, suggesting that it plays a role in fungal phenylalanine metabolism.

► We discovered the phenyllactate-producing yeast Wickerhamia fluorescens TK1. ► Phenylpyruvate reductase was purified from the yeast's cell-free extracts. ► The phenylpyruvate reductase uses NADPH, phenylpyruvate, glyoxylate and hydroxypyruvate as substrates. ► Its encoding gene was cloned. ► The protein constitutes a novel group of glyoxylate/hydroxypyruvate reductases.

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