Add time:08/22/2019 Source:sciencedirect.com
The separation of outer and cytoplasmic membrane fractions has been difficult to achieve with pseudomonads. A new procedure is described in which Pseudomonas facilis is treated with methyl picolinimidate, subsequently with buffered sucrose, and finally with lysozyme-EDTA to yield spheroplasts; spheroplast-derived envelopes can then be resolved by density gradient centrifugation into several components only one of which is more dense in the presence of Ni2+. This latter component, corresponding to the cell wall fraction, contains about 2% of the succinate dehydrogenase, a cytoplasmic membrane marker. Analogous experiments with erythrocytes establish that spectrin, a protein known to be located on the inner membrane surface, is not labeled by the methyl picolinimidate.
We also recommend Trading Suppliers and Manufacturers of METHYL PICOLINIMIDATE (cas 19547-38-7). Pls Click Website Link as below: cas 19547-38-7 suppliers
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View