Add time:08/22/2019 Source:sciencedirect.com
Cyclopenin (cas 19553-26-5) m-hydroxylase transforms cyclopenin, one of the two major alkaloids of Penicillium cyclopium, into cyclopenol. The enzyme belongs to the group of mixed function oxygenases. It needs molecular oxygen and a hydrogen donor (NAD(P)H, ascorbic acid, tetrahydropteridine) as cosubstrates, and it is inhibited by CN− and SCN− but not by CO. Inhibition by dicoumarol indicates that it may be a flavoprotein. With the exception of the alkaloid viridicatin, all tested compounds structurally related to cyclopenin are hydroxylated. The hydroxylase activity is measurable in the cultures at the end of the growth phase, i.e. during the period of alkaloid metabolism.
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