Add time:08/30/2019         Source:sciencedirect.com

“A sulfated polysaccharide from Monostroma nitidum Wittrock (Ulvophyceae)” was purified by anion-exchange and gel filtration column chromatographies. The isolated polysaccharide consisted of large amount of l-rhamnose with small amount of d-glucose, and it was regarded to be a rhamnan (cas 111018-90-7) sulfate (RS). Methylation analysis of the native and desulfated polysaccharide suggested that this polymer was mainly composed of 1,2- and 1,3-linked rhamnose residues with a ratio of ca 1:2. In addition, it was found the presence of 1,2,3-linked rhamnose and 1,4-linked glucose residues. Sulfate groups were suggested to be mainly located at C-2 and C-3 of 1,3- and 1,2-linked rhamnose residues, respectively. NMR analyses including 1D and 2D experiments indicated that RS consisted of sugar linkage units as follows:1: →3)-α-l-Rha-(1 → 3)-α-l-Rha-(1→2: →3)-α-l-Rha-(1 → 2)-α-l-Rha-(1→3: →3)-α-l-Rha-(1 → 3)-α-l-Rha-(1→2β-D-Glc-(1-↑

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