Add time:08/27/2019         Source:sciencedirect.com

With this study, we aimed to isolate the most promising bacteria to enhance persistent environmental pollutants (AROCLOR (cas 12767-79-2) 1242) phytoremediation from Salix. matsudana f. pendula Schneid, which has wide distribution and adaptability characteristics. A total of 12 endophytic bacteria strains were isolated and purifed from both Salix. matsudana f. pendula Schneid and its tissue culture seedlings. Of which, the strains CGL-1, SGL-1, SPL-3, 8, 17 and 24 were further isolated by enrichment culture with Aroclor 1242 as the sole source of carbon and energy. Compared with the growth characteristic of these strains, strain CGL-1 growth well under higher concentration of Aroclor 1242 exposure than others. The strain CGL-1 was identified as Enterobacter sp. with respect to 16S ribosomal DNA sequences, colony morphology and cell morphological characteristics. The optimum culture condition of the CGL-1 for removing Aroclor 1242 was optimized as: 27–32 °C, pH 7.0, NaCl ≦ 1%, inoculum 10%. Under these conditions, the removing ratio could reach to 43.2% after 7 d. The strain CGL-1 appears to be the most promising bacterium to enhance Aroclor 1242 phytoremediation. The endophytic bacterium is potentially useful for helping plants to tolerate pollutant stress and for increasing bioremediation resources.

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