Add time:08/24/2019         Source:sciencedirect.com

Endogenous scopolin and its aglycon scopoletin were tested in various plant cell suspensions by HPLC. The results indicate that the levels of these compounds varied greatly from one plant cell suspension to another (10−3–5 mg/g dry mass). Both scopoletin and scopolin were detected in the Nicotiana tabacum L. cell suspensions while only scopolin was detected in the Duboisia myoporoides R.Br., Solanum aviculare Forst. and Papaver somniferum L. cells. The identity of this scopolin was confirmed by NMR and MS data. Neither of these compounds was accumulated in the Nicotiana plumbaginifolia Viv. cell suspensions. Moreover, all the cell suspensions, except N. tabacum could bioconvert exogenous scopoletin to scopolin. The yield of the bioconversion varied from one cell suspension to another (21–92%). No correlation could be established between the capacity of the cells to bioconvert scopoletin to scopolin and the endogenous scopolin level.

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