Add time:08/26/2019         Source:sciencedirect.com

The mono- (2) and bis-phosphate (3) derivatives of d-threo-2,5-hexodiulose (1) (5-keto-d-fructose) were synthesized enzymically and purified by anion-exchange chromatography. The proportions, sizes of ring, and anomeric configurations were determined by F.t. 31P- and 13C-n.m.r. spectroscopy. Compound 2 was found to exist preponderantly (70–78%) in the β-pyranose form with the remainder existing in the 2R,5R-furanose form. Compound 3 assumes two different furanose forms in solution, one (77–84%) being the 2R,5R-furanose form and the other the 2S,5R-furanose form.

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