Add time:08/25/2019         Source:sciencedirect.com

Two complexes of bovine seminal ribonuclease with dinucleotides, uridylyl(2′-5′)adenosine (UpA) and 2′-deoxycytidylyl(3′-5′)-2′-deoxyadenosine (d(CpA)), were crystallized under unusual conditions involving a liquid-liquid phase separation. This phenomenon was induced by adding small aliquots of organic cosolutes to highly concentrated ammonium sulfate solutions. The liquid-liquid interface acts as a source of nucleation centers for growth of large crystals. Among the cosolutes tested in these salt-mediated crystallizations, 2-methyl-2,4-pentanediol was found to be the most efficient.

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