Add time:08/29/2019         Source:sciencedirect.com

Using a standard and modified europium chelate (Eu(TTA)3) and fluorescent brightener (FB) staining and microscopy procedure the suitability of the technique for studying the morphology of terrestrial and aquatic microorganisms was investigated. Cells of bacteria, yeasts, algae and protozoa were clearly distinguished not only by size but by characteristic morphology. Filaments and spores of algae, and mycelium and spores of fungi and actinomycetes were observed. Phase contrast microscopy of unstained preparations of most of these types of organisms revealed similar structures and indicated that the stain did not produce artifacts. The degree of visibility of cell contents depended upon the exposure lime of preparations to the two components of the (Eu(TTA)3) and FB stain and upon the wavelength of light under which microscopy was carried out. Adjustments to staining or observation conditions improved the resolution of cells and revealed more details of their structure. The staining procedure had no influence on the “normal” appearance of microorganisms. Any “abnormal” morphological feature was caused by the properties of the growth environment

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