Add time:08/27/2019         Source:sciencedirect.com

A simple and rapid gas chromatographic method is described for the simultaneous determination of protein and non-protein amino acids present in aqueous samples. The amino acids were converted into their N(O,S)-isobutoxycarbonyl methyl ester derivatives and measured by gas chromatography7 equipped with a split inlet mode, an electronic pressure control system and a hydrogen flame ionization detector using a DB-17ht capillary column. Using this method, the 21 protein amino acids and the 33 non-protein amino acids were quantitatively and reproducibly resolved within 25 min. The calibration curves were linear in the range 0.2–20 μg of each amino acid, with correlation coefficients being above 0.998, and the relative standard deviations in each point ranged from 0.2 to 9.2%. The detection limits of amino acids were 0.09–1.15 ng per injection.

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