Add time:07/14/2019         Source:sciencedirect.com

The preparation and antigenic property of 3-dehydrolithocholyl-glycine-bovine serum albumin(BSA) conjugate in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-3 position on the steroid nucleus is described. Antibody raised against antigen in the rabbit possessed high titer and specificity to lithocholylglycine, exhibiting no significant cross-reaction with free lithocholic acid or lithocholyltaurine.

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