Add time:08/30/2019         Source:sciencedirect.com

A method for the determination of the sodium salt of 5-iodosalicyhc acid (5-I-S-Na) and total iodine is described. The method for the determination of 5-T-S-Na is based on the ultraviolet light absorption of this substance. Control experiments with the method for the determination of 5-1-S-Na and total iodine showed a satisfactory recovery for added 5-iodosalicyhc acid (5-I-S). The boiling of the solution with hydrochloric acid necessary for the hydrolysis of conjugated 5-I-S caused only a slight loss of this acid. It was found that the values for 5-I-S correctly represent this substance, and that only about 3% of this acid was present in a free state. The rest was in a conjugated form. The presence in the urine of an excess of iodine, as compared with total 5-I-S, indicated the presence of metabolites of this acid. Inorganic iodine was not found.

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