Add time:09/03/2019         Source:sciencedirect.com

Flavonoids are commonly occurring polyphenolic compounds with a wide array of bioactive properties, including its positive role on osteoblast differentiation and bone formation. Their profound ability to chelate various metal ions supported its candidature as a novel biomaterial for tissue engineering applications. We aimed to investigate the role of Morin-zinc complexes (Morin mono complex, [Zn(morin)(H2O)] and Morin bis complex, [Zn(morin)2]) in promoting osteoblast differentiation at both molecular and cellular levels. In vitro biocompatibilities of the synthesized complexes (20–200 μM) were assessed with MG-63, H9c2 and C3H10T1/2 cells and chick embryo model was used to assess in vivo toxicity. Both Morin and Morin zinc complexes were found to be non-toxic at 60μM concentration. Furthermore, at cellular level, the complexes increased alkaline phosphatase activity (ALP) and calcium deposition. At molecular level it increased Runx2, type 1 collagen mRNAs expression and enhanced the secretion of osteocalcin (OC) and osteonectin (ON) proteins. Among the synthesized complexes, [Zn(morin)2] exhibited more pronounced effects compared to naïve Morin and [Zn(morin)] complex. Overall, the current study proposes that Morin and Morin zinc complexes could be used for bone tissue engineering applications.

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