Add time:09/01/2019         Source:sciencedirect.com

Procedures for the separation and quantitative determination of 3,4-Dimethoxyphenethylamine (cas 120-20-7) as the isothiocyanate derivative are given and the thin-layer chromatographic properties of 3,4-dimethoxyphenethylamine isothiocyanate are described. The sensitivity of this method for qualitative identification on thin-layer chromatography is 0.003 μg and for quantitative determination 0.01 μg. Recovery experiments were carried out with varying quantities of 3,4-dimethoxyphenethylamine added to 80% of a 24-h urine collection. Levels as low as 5 μg of 3,4-dimethoxyphenethylamine added to a urine sample yielded a distinct 3,4-dimethoxyphenethylamine isothiocyanate spot on thin-layer chromatography when 10% of an aliquot of the final concentrate was spotted.

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