Add time:09/05/2019         Source:sciencedirect.com

1.1. Further data are reported on the fractionation of brewers' yeast and Escherichia coli amino-acyl transfer RNA (s-RNA) by chromatography on heated DEAE-cellulose columns and by chromatography on Sephadex G-25 partition columns.2.2. Elution profiles of brewers' yeast and E. coli s-RNA from both DEAE-cellulose and Sephadex partition columns are compared. Considerable differences were found in the elution profiles for yeast and E. coli s-RNA on DEAE-cellulose columns.3.3. Elution positions of species of s-RNA accepting single amino acids were determined and the order of elution of individual species of s-RNA was compared. The elution positions and the order of elution differed in the two procedures.4.4. With these methods species of s-RNA accepting a single amino acid have been purified, and major and minor s-RNA species accepting a single amino acid have been detected.5.5. The elution positions and hence properties of yeast s-RNA species accepting a particular amino acid often differ from those of E. coli s-RNA species accepting the same amino acid.6.6. The number of yeast s-RNA species accepting a particular amino acid often differs from that found for E. coli. This confirms earlier reports of species specificity of s-RNA.

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