Add time:08/30/2019         Source:sciencedirect.com

Natural pyrethrins accumulate to high concentrations in the flower achenes of pyrethrum (Tanacetum cinerariifolium). They are extracted from dried flowers and widely used as a natural pesticide. Chrysanthemol synthase (CHS) is the first key enzyme in the biosynthesis of pyrethrins. In this work, a 1128 bp TcCHS promoter fragment was cloned from pyrethrum genomic DNA. The sequence contained cis-elements predicted to be responsive to different hormones, light, and environmental stresses. To characterize the promoter it was fused to the reporter genes green fluorescent protein (GFP) and β-glucuronidase (GUS), and respectively transformed into florist's chrysanthemum (Chrysanthemum morifolium ‘1581’) and tobacco (Nicotiana tabacum). GFP fluorescence in florist's chrysanthemum ‘1581’and GUS staining of tobacco showed that the TcCHS promoter was exclusively expressed in the glandular secretory trichomes (GSTs) of both plant species. The findings will support research on factors influencing the accumulation of pyrethrins, and can be used for trichome-specific metabolic engineering of plants to ensure minimal adverse effects on plant growth and development.

We also recommend Trading Suppliers and Manufacturers of CHRYSANTHEMYL ALCOHOL (cas 18383-59-0). Pls Click Website Link as below: cas 18383-59-0 suppliers