Add time:07/17/2019         Source:sciencedirect.com

The reduced form of longer-wavelength cytochrome b (b566) was identified in rat liver mitochondria in State 5 on addition of ferricyanide. Addition of menadione caused oxidation of b566 and reduction of cytochrome c1 (+ c). In the presence of menadione, ferricyanide induced reduction of b566 and oxidation of cytochrome c1 + c, and exhaustion of the added ferricyanide resulted in oxidation of b566 and reduction of cytochrome c1 + c.Antimycin A enhanced the extent of the ferricyanide-dependent redox change of b566 from 20 to 68% of the total absorbance at 566 nm.Uncouplers hardly affected the change of b566 in the presence of antimycin A, but lowered the reduced level in the absence of antimycin A.

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