Add time:08/31/2019 Source:sciencedirect.com
Diazomethylation esterified all tested acidic indoles without appreciable loss. A group of ten methyl ester derivatives were obtained in crystalline form. These were used to test the relative values of four gas chromatographic substrates. Quantitative comparisons led to the selection of Versamid 900, HI-EFF 8 BP, and SE-30 as permitting complete separation of all indole esters, while SE-52 was unsatisfactory. Gradient elution column and thin-layer chromatography as pre- and post-purification techniques for these indole esters were also compared, as was the usefulness of UV spectroscopy and spectrophotofluorometry in acidic indole analysis.
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