Add time:09/10/2019         Source:sciencedirect.com

The combined effects of perfluorocarbon and glucose concentrations on actinorhodin (cas 1397-77-9) production by Streptomyces coelicolor A3(2) were studied in a 2-l bioreactor using response surface methodology. A 22 full-factorial central composite design was employed for experimental design and analysis of the results. The optimum PFC and glucose concentrations were found to be 42.7% and 12.25 g/l, respectively. In these conditions, actinorhodin concentration of 60 mg/l with a biomass concentration of 1.9 g/l, a maximum volumetric oxygen uptake rate of 180 mgO2/l h and a glucose consumption rate of 0.110 g glucose/l h was attained. These results are in close agreement with the model predictions.

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