Add time:09/01/2019         Source:sciencedirect.com

radicinin (cas 1402-20-6) is a phytotoxic and antibiotic metabolite produced by some phytopathogenic fungi. Precursor administration and cell-free experiments with deoxyradicinin and radicinin were carried out in Bipolaris coicis H13-3. When deoxyradicinin was administered to the fungus, radicinin and 3-epi-radicinin formed. When radicinin administered, 3-epi-radicinin was formed. Their formation was confirmed by cell-free experiments. Deoxyradicinin 3-monooxygenase which catalyzes conversion of deoxyradicinin to radicinin showed the best activity at 35 °C and pH 7.0, and required NAD+ as co-enzyme. Its molecular weight was determined to be 130–184 kDa. Radicinin epimerase catalyzing the reaction of radicinin to 3-epi-radicinin was purified from a cell-free extract. Radicinin epimerase is a homodimer of a 28 kDa subunit, and its highest activity was achieved at 30–35 °C and pH 7.0–9.0.

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