Add time:09/03/2019         Source:sciencedirect.com

The rapidly labelled 35 to 45-S RNA of L cells is replaced during a chase period in the presence of actinomycin by 30-S and 20-S ribosomal sizes. This process takes place in the nucleus and most of the RNA remains within the nucleus for up to 4 h. At the same time some labelled 20-S, but not 30-S, RNA appears in the cytoplasm.In cells infected with encephalomyocarditis virus the amount of 32P found in nuclear 35 to 45-S RNA after a 30-min pulse quickly falls below that in normal cells. On adding actinomycin to stop further RNA synthesis the pulse-labelled RNA is not destroyed but is converted to 30-S and 20-S RNA just as in uninfected cells. Therefore the subnormal labelling after infection is probably due to inhibition of RNA synthesis rather than to an enhanced specific breakdown of rapidly synthesised RNA.

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