Add time:09/06/2019         Source:sciencedirect.com

A simple and cost effective unsymmetrical azine based Schiff base, 5-diethylamino-2-[(2-hydroxy-benzylidene)hydrazonomethyl]-phenol (1) was synthesized which selectively detect Cu2 + ions in the presence of other competitive ions through “naked eye” in physiological conditions (EtOH–buffer (1:1, v/v, HEPES 10 mM, pH = 7.4)). The presence of Cu2 + induce color change from light yellow green to yellow with the appearance of a new band at 450 nm in UV–Vis spectra of Schiff base 1. The fluorescence of Schiff base 1 (10 μM) was quenched completely in the presence of 2.7 equiv. of Cu2 + ions. Sub-micromolar limit of detection (LOD = 3.4 × 10− 7 M), efficient Stern–Volmer quenching constant (KSV = 1.8 × 105 L mol− 1) and strong binding constant (log Kb = 5.92) has been determined with the help of fluorescence titration profile. Further, 1 − Cu2 + complex was employed for the detection of phosphate ions (PO43 −, HPO42 − and H2PO4−) at micromolar concentrations in EtOH–buffer of pH 7.4 based on fluorescence recovery due to the binding of Cu2 + with phosphate ions. Solubility at low concentration in aqueous medium, longer excitation (406 nm) and emission wavelength (537 nm), and biocompatibility of Schiff base 1 formulates its use in live cell imaging.

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