Add time:09/03/2019         Source:sciencedirect.com

Centella asiatica (L.) Urban is a tropical medicinal plant with a long history of therapeutic uses. Madecassic acid (cas 18449-41-7) and terminolic acid, a pair of structural isomers, are two constituents of Centella asiatica. A method using reversed-phase high performance liquid chromatography in which β-cyclodextrin (β-CD) was the additive in mobile phase has been developed for separation and determination of the structural isomers. The two compounds can be isolated with high resolution on a C18 reversed-phase column with the addition of β-CD in the mobile phase. The separation mechanism of the isomers was discussed. It was assumed that the separation of the isomers might have been resulted from different inclusion forces of complexes with β-CD. The effects of β-CD concentration and the pH of mobile phase on resolution were investigated. It was found that the resolution of the isomers increased with the increase in β-CD concentration when the mobile phase consisted of methanol-water (65:35, v/v) at pH 4. The correlation coefficient (γ2) of the linear calibration curve between the peak area and concentration of madecassic acid was 0.9989 in the range of 0.1–5.0 g/L. This method was successfully used to determine the madecassic acid in triterpenic genins of Centella asiatica.

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