Add time:09/05/2019         Source:sciencedirect.com

Publisher SummaryThis chapter discusses the preparation of d-altro-heptulose (d-sedoheptulose) 7-phosphate. The important metabolite d-sedoheptulose (d-altro-heptulose) 7-phosphate has been prepared by biological and chemical procedures. In general, the biological syntheses are comparatively lengthy and cumbersome although the separation of sedoheptulose 7-phosphate from the reaction mixture takes somewhat less time. The chemical synthesis is very fast, yields are comparable to those for the best biological syntheses, and the product is essentially pure. For preparation, barium d-ribose 5-phosphate trihydrate is dissolved in 130 ml of cold 0.01 N hydrobromic acid, the solution is treated with 66 ml of cold 0.52 N sodium sulfate, and barium sulfate is removed by centrifugation. The precipitate is washed once with 12 ml of cold 0.01 N hydrobromic acid, and the washing is combined with the original, supernatant liquor.

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