Add time:09/03/2019         Source:sciencedirect.com

As an extension of previous methods for enantiomer analyses of diacylglycerols, a highly sensitive HPLC method was developed for the determination of the absolute configuration and optical purity of monoacylglycerols. Chiral derivatization by a fluorescent (S)-TBMB carboxylic acid followed by a normal-phase HPLC separation of the derived diastereomeric di-(S)-TBMB-carbonyl-sn-1- and -sn-3-monoacylglycerols provided useful tools to determine the chirality of a series of saturated and unsaturated monoacylglycerols (C12:0C18:0, C18:1, C18:2 and C18:3) [(S)-TBMB = (S)=(+)-2-tert.-butyl-2-methyl-1,3-benzodioxole]. In addition, the HPLC elution times of each diastereomeric isomer were correlated with the chain length (carbon number) and the double bond numbers of acyl groups.

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