Add time:09/03/2019         Source:sciencedirect.com

The hydrolysis of the p-anisoyl group from the active centre of the thrombolytic proenzyme APSAC has been studied in human plasma, whole blood and thrombin-induced plasma clots using a radiochemical method based on a tritiated acyl group and in human plasma by a fibrinolytic method which exploited the inertness of acyl enzymes towards plasma inhibitors. In human plasma and blood, a half-life for deacylation of approximately 105–120 min independent of agent concentration, was found. The deacylation half-life was not significantly altered by the presence of human fibrin in either lysing or stable plasma clots nor was it changed in the presence of specific human antistreptokinase antibody

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