Add time:07/14/2019         Source:sciencedirect.com

An analytical system to quantify agaritine present in Agaricus blazei, Agaricus bisporus, and other food mushrooms was established using high pressure liquid chromatography combined with mass-spectroscopy (HPLC–MS). Water extracts from dried, homogenised mushrooms were kept at differing temperatures for defined periods to investigate the heat-stability of agaritine. Homogenates were then freeze-dried, and agaritine was extracted using methanol. After evaporation of methanol, agaritine levels were analysed by HPLC–MS. A. bisporus contained 341 ± 32 μg/g agaritine, and A. blazei contained 22–57 μg/g agaritine. While pure agaritine in H2O solution was heat-unstable and decomposed exponentially at 120 °C, agaritine in Agaricus water extracts was partially heat stable, and 20–50% of agaritine remained after 120 min at 120 °C. Thus agaritine, a known carcinogen, is likely to be present in Agaricus extracts sold as nutritional supplements. Therefore, a method was developed that can be used to remove agaritine from water extracts in order to prevent health risk. Agaritine was successfully removed from Agaricus water extracts by ethanol fractionation.

► Agaricus blazei and Agaricus bisporus contain agaritine which is a carcinogen. ► Agaritine contained in Agaricus water extracts is partially heat stable. ► Removal of agaritine from water extracts is carried out by ethanol fractionation. ► Analytical system (HPLC–MS) to quantify agaritine in mushrooms was established.

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