Add time:09/04/2019         Source:sciencedirect.com

3′Cytidylic acid was irradiated with ultraviolet light to produce as the main photoproduct C*p.† The absorption spectrum of C*p has maxima at 240 and 210 mμ and has a pK of 5·56. It was separated from Cp by rapid high-voltage electrophoresis (20 minutes 0°C at pH 4·4). The separated C*p was shown to reverse to Cp and deaminate to U*p. The rate constants for reversal and deamination were measured at 6, 15 and 25°C over a pH range from 3 to 10. The reversal rate constant is maximum at pH 5·0 with a value of 1·6 × 10−2 min−1 at 25°C. The corresponding deamination rate constant is 4·0 × 10−4 min−1. The maximum amount of U*p which can be recovered from C*p is independent of temperature and varies from 2·5 to 14% over the pH range, with the minimum value at pH 5·0. The experiments provide good evidence that the photoproduct C*p is the water adduct of Cp. The implications of these findings on code changes in UNA and DNA are discussed.

We also recommend Trading Suppliers and Manufacturers of CYCLIC CYTIDYLIC ACID SODIUM SALT (cas 15718-51-1). Pls Click Website Link as below: cas 15718-51-1 suppliers