Add time:09/04/2019 Source:sciencedirect.com
Iron(lll)-loaded 8-quinolinol silica gel (QSG) is shown to be an effective stationary phase for the high-performance liquid chromatographic separation of phenols, including EPA Priority Pollutant species. The QSG itself exhibits significant reversed-phase and hydrogen-bonding interactions with sample compounds, but the presence of the iron(III) on the QSG substantially increases both retention and resolution. Very good column stability is observed, with no bleeding of chelated metal ion. Therefore, the presence of iron(III) in the mobile phase is not necessary. Preferred column conditions are a water—acetonitrile mobile phase buffered at pH 4 with acetic acid—sodium acetate. A study of retention volumes as a function of pH suggests that acetate species competitively interact with stationary phase sites.
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