Add time:09/06/2019         Source:sciencedirect.com

Formation and inhibition of malonaldehyde (MA) from blood plasma lipids oxidized by Fenton's reagent in the absence or presence of probucol [4,4′-(isopropylidenedithio)bis(2,6-di-tert-butylphenol)] and l-ascorbic acid were investigated. The amount of MA formed was quantitatively analysed by gas chromatography. l-Ascorbic acid inhibited MA formation by about 30% at the level of 4.0 μmol, but the amount of MA formed was increased by the presence of probucol. When 3.0 μmol oxidized probucol was hydrolysed at pH 1, 3 and 5, 2616.5 nmol, 287.5 nmol and 103.9 nmol MA were recovered, respectively. This is the first report of quantitative analysis of MA formed from probucol on oxidation.

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