Add time:09/24/2019         Source:sciencedirect.com

Monodisperse, millimeter-sized, lipase-entrapped NIPA-co-PEGMEA (N-isopropylacrylamide-co-poly(ethylene glycol) methyl ether acrylate) gel beads were prepared by sedimentation polymerization. The feasibility of the lipase-entrapped gel for use in biodiesel fuel (BDF) production was evaluated by studying its catalytic performance in transesterification of TRIOLEIN (cas 122-32-7) with ethanol as the model. The reaction rate was expressed by the ping-pong bi-bi mechanism with ethanol inhibition; the parameters for the immobilized lipase were as follows; the maximum reaction rate, Vmax, was 0.405 mol/(min kg − Lipase PS); the Michaelis constants for triolein and ethanol, Km,T and Km,EtOH, were 481 and 1770 mol/m3, respectively; the ethanol inhibition constant, Ki, was 394 mol/m3. The NIPA-co-PEGMEA gel support improved the stability of the immobilized lipase. We developed a water-washing process wherein the by-product glycerol, which is absorbed and accumulated in the NIPA-co-PEGMEA gel, is removed, so that the lipase-entrapped gel could be reused for multiple cycles (10 cycles were confirmed). The glycerol absorbability of the NIPA-co-PEGMEA gel makes it promising for use in facile BDF production, as it allows for the simultaneous enzymatic reaction and glycerol removal.

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